Visible spectrophotometric and first-derivative UV spectrophotometric determination of rifampicin and isoniazid in pharmaceutical preparations

Autor:	Maria Inês Rocha Miritello Santoro, S A Benetton, V M Borges, Erika Rosa Maria Kedor-Hackmann
Rok vydání:	1998
Předmět:	Chromatography medicine.diagnostic_test Chemistry Isoniazid Buffer solution bacterial infections and mycoses Dosage form Analytical Chemistry Absorbance chemistry.chemical_compound Spectrophotometry medicine Derivatization Quantitative analysis (chemistry) Rifampicin medicine.drug
Zdroj:	Talanta. 47:639-643
ISSN:	0039-9140
DOI:	10.1016/s0039-9140(98)00111-8
Popis:	Two methods are described for the determination of rifampicin and isoniazid in mixtures by visible spectrophotometry and first-derivative ultraviolet spectrophotometry. The absorbance at 475 nm in buffer solution pH 7.4 was employed to determine rifampicin after applying the three-point correction technique between 420 and 520 nm, while the amplitude of the first-derivative spectrophotometric spectrum at 257 nm in HCl 0.012 M was selected for the determination of isoniazid. The methods are rapid, simple and do not require any separation step. The recovery average was 99.03% for rifampicin and 100.01% for isoniazid. The methods were applied to determine the two compounds in commercial capsules and compared with the official method of the USP XXIII with good agreement between the results.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b12706291e7c091e67375492ccc26ea https://doi.org/10.1016/s0039-9140(98)00111-8 Zobrazit plný text záznamu Full Text from ScienceDirect