Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance
| Autor: | Tiancong Qi, Julie Pham, Clara Youngna Lee, Priscila Giuseppe, Arturo Ortega, Kyungyong Seong, Rebecca Mackelprang, Myeong-Je Cho, Daniela P. T. Thomazella, Yu Geng, Samuel F. Hutton, Brian J. Staskawicz, Upinder S. Gill, D. Dahlbeck |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
CRISPR/Cas9 technology
0106 biological sciences 0301 basic medicine Gentisates Mutant Sequence Homology 01 natural sciences Solanum lycopersicum Gene Expression Regulation Plant Arabidopsis 2.1 Biological and endogenous factors Plant Immunity Aetiology Pathogen Phylogeny Disease Resistance Plant Proteins Genetics Oomycete Multidisciplinary biology food and beverages Plants Biological Sciences Plants Genetically Modified Up-Regulation Amino Acid Infectious Diseases CRISPR Infection Salicylic Acid Biotechnology disease resistance Xanthomonas DMR6 salicylic acid Genetically Modified Plant disease resistance Cas9 technology 03 medical and health sciences crop engineering Gene Plant Diseases Sequence Homology Amino Acid Arabidopsis Proteins Plant biology.organism_classification Emerging Infectious Diseases 030104 developmental biology Gene Expression Regulation Mutation Biocatalysis Subfunctionalization Antimicrobial Resistance Transcriptome Function (biology) 010606 plant biology & botany |
| Zdroj: | Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 27 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Plant diseases are among the major causes of crop yield losses around the world. To confer disease resistance, conventional breeding relies on the deployment of single resistance (R) genes. However, this strategy has been easily overcome by constantly evolving pathogens. Disabling susceptibility (S) genes is a promising alternative to R genes in breeding programs, as it usually offers durable and broad-spectrum disease resistance. In Arabidopsis, the S gene DMR6 (AtDMR6) encodes an enzyme identified as a susceptibility factor to bacterial and oomycete pathogens. Here, we present a model-to-crop translational work in which we characterize two AtDMR6 orthologs in tomato, SlDMR6-1 and SlDMR6-2. We show that SlDMR6-1, but not SlDMR6-2, is up-regulated by pathogen infection. In agreement, Sldmr6-1 mutants display enhanced resistance against different classes of pathogens, such as bacteria, oomycete, and fungi. Notably, disease resistance correlates with increased salicylic acid (SA) levels and transcriptional activation of immune responses. Furthermore, we demonstrate that SlDMR6-1 and SlDMR6-2 display SA-5 hydroxylase activity, thus contributing to the elucidation of the enzymatic function of DMR6. We then propose that SlDMR6 duplication in tomato resulted in subsequent subfunctionalization, in which SlDMR6-2 specialized in balancing SA levels in flowers/fruits, while SlDMR6-1 conserved the ability to fine-tune SA levels during pathogen infection of the plant vegetative tissues. Overall, this work not only corroborates a mechanism underlying SA homeostasis in plants, but also presents a promising strategy for engineering broad-spectrum and durable disease resistance in crops. |
| Databáze: | OpenAIRE |
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