Regulation of collagen synthesis by ascorbic acid: Characterization of the role of ascorbate-stimulated lipid peroxidation
| Autor: | Joel S. Gordon, Richard A. Berg, L. J. Hendricks, P.A. Falkenstein, Jeffrey C. Geesin |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Antioxidant
medicine.medical_treatment Biophysics Ascorbic Acid Biochemistry Antioxidants Superoxide dismutase Lipid peroxidation chemistry.chemical_compound Benzene Derivatives medicine Humans Fibroblast Molecular Biology Cells Cultured Chelating Agents biology Dimethyl sulfoxide Free Radical Scavengers Ascorbic acid medicine.anatomical_structure chemistry Catalase biology.protein lipids (amino acids peptides and proteins) Collagen Lipid Peroxidation Mannitol medicine.drug |
| Zdroj: | Archives of Biochemistry and Biophysics. 290:127-132 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(91)90598-d |
| Popis: | Recently, we have described the ability of traditional lipid peroxidation inhibitors to inhibit ascorbate-stimulated collagen synthesis. In order to characterize further this effect, we have tested the ability of known and potential inhibitors of lipid peroxidation for their effects on ascorbate-stimulated collagen synthesis and lipid peroxidation. In our experiments, mannitol, a water soluble antioxidant, had no effect on ascorbate-induced collagen synthesis nor on lipid peroxidation. However, alpha-tocopherol, which is a lipophilic antioxidant, inhibited both effects of ascorbate. Superoxide dismutase, catalase, and their polyethylene glycol conjugate forms did not inhibit the ascorbate-stimulated collagen synthesis or lipid peroxidation. In addition, no effect was seen with the oxygen radical scavengers isopropanol, ethanol, or dimethyl sulfoxide. Two iron chelators, o-phenanthroline and alpha,alpha-dipyridyl, both inhibited ascorbate-induced lipid peroxidation and collagen synthesis, consistent with the previously described iron-dependence of lipid peroxidation by ascorbate. These results support a correlation between collagen synthesis and lipid peroxidation and provide a theory for the mechanism of ascorbic acid regulation of collagen synthesis. |
| Databáze: | OpenAIRE |
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