Novel Mutations Associated with Resistance to Bacillus sphaericus in a Polymorphic Region of the Culex quinquefasciatus cqm1 Gene
| Autor: | Tatiany Patrícia Romão, Maria Helena Neves Lobo Silva-Filha, Osvaldo Pompílio de-Melo-Neto, Karlos Diogo de Melo Chalegre, Eloína Maria de Mendonça Santos, Cláudia Maria Fontes de Oliveira, Daniella Aliny Tavares, Lígia Maria Ferreira |
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| Rok vydání: | 2012 |
| Předmět: |
Sequence analysis
Bacterial Toxins Bacillus Receptors Cell Surface medicine.disease_cause Applied Microbiology and Biotechnology Bacillus sphaericus chemistry.chemical_compound Invertebrate Microbiology medicine Animals Allele Receptor Gene Alleles Genetics Polymorphism Genetic Ecology biology Toxin fungi biology.organism_classification Molecular biology Culex quinquefasciatus Culex chemistry Mutation Insect Proteins Brazil DNA Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 78:6321-6326 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.01199-12 |
| Popis: | Bin toxin from Bacillus sphaericus acts on Culex quinquefasciatus larvae by binding to Cqm1 midgut-bound receptors, and disruption of the cqm1 gene is the major cause of resistance. The goal of this work was to screen for a laboratory-selected resistance cqm1 REC allele in field populations in the city of Recife, Brazil, and to describe other resistance-associated polymorphisms in the cqm1 gene. The cqm1 REC allele was detected in the four nontreated populations surveyed at frequencies from 0.001 to 0.017, and sequence analysis from these samples revealed a novel resistant allele ( cqm1 REC-D16 ) displaying a 16-nucletotide (nt) deletion which is distinct from the 19-nt deletion associated with cqm1 REC . Yet a third resistant allele ( cqm1 REC-D25 ), displaying a 25-nt deletion, was identified in samples from a treated area exposed to B. sphaericus . A comparison of the three deletion events revealed that all are located within the same 208-nt region amplified during the screening procedure. They also introduce equivalent frameshifts in the sequence and generate the same premature stop codon, leading to putative transcripts encoding truncated proteins which are unable to locate to the midgut epithelium. The populations analyzed in this study contained a variety of alleles with mutations disrupting the function of the corresponding Bin toxin receptor. Their locations reveal a hot spot that can be exploited to assess the resistance risk through DNA screening. |
| Databáze: | OpenAIRE |
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