Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: Cloning, expression and phylogenetic relationships

Autor: Jacqueline Grima-Pettenati, Deborah Goffner, Joël Piquemal, Jan Van Doorsselaere, Odile Poeydomenge, Alain M. Boudet, Simon Hawkins, Eric Lacombe
Přispěvatelé: Unité de recherche Amélioration, Génétique et Physiologie Forestières (AGPF), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Předmět:
0106 biological sciences
DNA
Complementary
Sequence analysis
Molecular Sequence Data
Plant Science
Biology
Reductase
Lignin
01 natural sciences
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Oxidoreductase
Complementary DNA
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
Escherichia coli
Genetics
Animals
Humans
Amino Acid Sequence
Cloning
Molecular
Gene
Peptide sequence
In Situ Hybridization
Phylogeny
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Mammals
chemistry.chemical_classification
Eucalyptus
0303 health sciences
Plants
Medicinal
Base Sequence
Sequence Homology
Amino Acid
integumentary system
BIOCHIMIE
fungi
food and beverages
Cell Biology
biology.organism_classification
Aldehyde Oxidoreductases
Recombinant Proteins
Eucalyptus gunnii
Biochemistry
chemistry
Multigene Family
Cinnamoyl-CoA reductase
010606 plant biology & botany
Zdroj: Scopus-Elsevier
Plant Journal
Plant Journal, Wiley, 1997, 11 (3), pp.429-441
ISSN: 0960-7412
1365-313X
Popis: Summary Cinnamoyl CoA:NADP oxidoreductase (CCR, EC 1.2.1.44) catalyzes the conversion of cinnamoyl CoA esters to their corresponding cinnamaldehydes, i.e. the first specific step in the synthesis of the lignin monomers. The cloning of a cDNA encoding CCR in Eucalyptus gunnii (EUCCR) is reported here. The identity of the EUCCR cDNA was demonstrated by comparison with peptide sequence data from purified CCR and functional expression of the recombinant enzyme in Escherichia coli. Sequence analysis revealed remarkable homologies with dihydroflavonol-4-reductase (DFR), the first enzyme of the anthocyanin biosynthetic pathway. Moreover, significant similarities were found with mammalian 3β-hydroxysteroid dehydrogenase and bacterial UDP-galactose-4-epimerase, suggesting that CCR shared a common ancestor with these enzymes and can therefore be considered as a new member of the mammalian 3β-hydroxysteroid dehydrogenase/ plant dihydroflavonol reductase superfamily. In Eucalyptus gunnii, CCR is encoded by one gene containing four introns whose positions are similar to those of introns I, II, III and V in DFR genes from dicots. In agreement with the involvement of CCR in lignification, the CCR transcript was shown to be expressed in lignified organs, i.e. root and stem tissues, and was localized mainly in young differentiating xylem. On the other hand, its abundance in Eucalyptus leaves suggests that monolignols may be precursors of end products other than lignins. This first characterization of a gene corresponding to CCR opens new possibilities to genetically engineer plants with lower lignin content. This is particularly important for woody plants such as Eucalyptus which are used for pulp making.
Databáze: OpenAIRE
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