Polymorphisms in major histocompatibility complex genes and its associations with milk quality in Murrah buffaloes
| Autor: | Marina R. S. Fortes, Marina Mortati Dias Barbero, D.J.A. Santos, Ana Cláudia de Freitas, Humberto Tonhati, Nedenia Bonvino Stafuzza |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), The University of Queensland |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Somatic cell count
Candidate gene Linkage disequilibrium Buffaloes 040301 veterinary sciences Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Major Histocompatibility Complex 0403 veterinary science Food Animals Genotype Animals Allele Gene Genetics Milk fat Haplotype 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Genotype frequency Bubalus bubalis Milk Haplotypes Milk protein Female Animal Science and Zoology MHC SNPs |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2019-10-06T16:42:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Animal breeding programs have used molecular genetic tools as an auxiliary method to identify and select animals with superior genetic merit for milk production and milk quality traits as well as disease resistance. Genes of the major histocompatibility complex (MHC) are important molecular markers for disease resistance that could be applied for genetic selection. The aim of this study was to identify single nucleotide polymorphisms (SNPs) and haplotypes in DRB2, DRB3, DMA, and DMB genes in Murrah breed and to analyze the association between molecular markers and milk, fat, protein and mozzarella production, fat and protein percentage, and somatic cell count. Two hundred DNA samples from Murrah buffaloes were used. The target regions of candidate genes were amplified by polymerase chain reaction (PCR) followed by sequencing and identification of polymorphisms. Allele and genotype frequencies, as well as linkage disequilibrium between SNPs, were calculated. Genotypes were used in association analyses with milk production and quality traits. Except for the DMA gene, identified as monomorphic, the other genes presented several polymorphisms. The DMB, DRB2, and DRB3 genes presented two, six, and seven SNPs, respectively. Fifty-seven haplotype blocks were constructed from 15 SNPs identified, which was used in association analyses. All the studied traits had at least one associated haplotype. In conclusion, it is suggested that the haplotypes found herein can be associated with important traits related to milk production and quality. Department of Animal Science School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP) Department of Exact Sciences School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP) School of Chemistry and Molecular Biosciences The University of Queensland, Saint Lucia Campus Department of Animal Science School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP) Department of Exact Sciences School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|