An Erythromycin Derivative Produced by Targeted Gene Disruption in Saccharopolyspora erythraea
| Autor: | K. B. Idler, J. O. Leung, Sue J. Swanson, J. M. Weber, James B. McAlpine |
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| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial DNA Mutational Analysis Molecular Sequence Data Restriction Mapping Mutant Mixed Function Oxygenases chemistry.chemical_compound Bacterial Proteins Cytochrome P-450 Enzyme System Biosynthesis Amino Acid Sequence chemistry.chemical_classification Multidisciplinary Bacteria Base Sequence biology Streptomycetaceae Cytochrome P450 Biological activity biology.organism_classification Erythromycin Enzyme chemistry Biochemistry Genes Bacterial biology.protein Saccharopolyspora erythraea Actinomycetales Plasmids |
| Zdroj: | Science. 252:114-117 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.2011746 |
| Popis: | Derivatives of erythromycin with modifications at their C-6 position are generally sought for their increased stability at acid pH, which in turn may confer improved pharmacological properties. A recombinant mutant of the erythromycin-producing bacterium, Saccharopolyspora erythraea, produced an erythromycin derivative, 6-deoxyerythromycin A, that could not be obtained readily by chemical synthesis. This product resulted from targeted disruption of the gene, designated eryF (systematic nomenclature, CYP107), that apparently codes for the cytochrome P450, 6-deoxyerythronolide B (DEB) hydroxylase, which converts DEB to erythronolide B (EB). Enzymes normally acting on EB can process the alternative substrate DEB to form the biologically active erythromycin derivative lacking the C-6 hydroxyl group. |
| Databáze: | OpenAIRE |
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