A set of canine interrepeat sequence PCR markers for high-throughput genotyping
| Autor: | Jerry Pelletier, Manjula Das, Hakan Sakul, Gregory M. Acland, Julius Kong |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Genetic Markers
Genetics Radiation Hybrid Mapping Polymorphism Genetic Genotype Genetic Linkage Physiology Interspersed repeat Biology biology.organism_classification Polymerase Chain Reaction law.invention Dogs Canis law Animals High throughput genotyping Radiation hybrid mapping Genotyping Conserved Sequence Polymorphism Single-Stranded Conformational Polymerase chain reaction Short Interspersed Nucleotide Elements Sequence (medicine) |
| Zdroj: | Physiological Genomics. 4:13-24 |
| ISSN: | 1531-2267 1094-8341 |
| DOI: | 10.1152/physiolgenomics.2000.4.1.13 |
| Popis: | One hundred and sixteen interspersed repetitive DNA sequence (IRS)-PCR markers have been developed and characterized from Canis familiaris for high-throughput filter-based genotyping. We present a detailed analysis of markers produced by amplification using primers directed to the conserved regions of the C. familiaris short interspersed nuclear element ( Can-SINE). The majority of IRS-PCR markers developed were moderately to highly polymorphic with mean heterozygosity (HET) and polymorphism information content (PIC) values of ∼0.6. The HET value for 22.3% of the markers exceeded 0.7. We also demonstrate that sequence variation of Can-SINEs between breeds is significant and also represents a rich source of polymorphisms. Mapping of 73 of the markers to the existing integrated linkage-radiation hybrid map enriches the map as well as establishes the utility of the markers. The significance and utility of this new class of IRS-PCR Can-SINE-based markers for high-throughput genotyping is discussed. This method can also be extended to other species that are currently map-poor but have a sufficiently high density of SINEs to allow IRS-PCR. |
| Databáze: | OpenAIRE |
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