Selection of reference genes for gene expression studies in astrocytomas
| Autor: | Dariusz J. Jaskólski, Sylwia M. Gresner, Wielisław Papierz, Pawel P. Liberski, Ewa Golanska, Dominika Kulczycka-Wojdala |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Ribosomal Proteins
Hypoxanthine Phosphoribosyltransferase Biophysics Astrocytoma Biochemistry Polymerase Chain Reaction law.invention Histones stomatognathic system law Reference genes Gene expression Humans POLR2A Molecular Biology Gene Glyceraldehyde 3-phosphate dehydrogenase Polymerase chain reaction Genetics biology Gene Expression Profiling Cell Biology Reference Standards Actins Housekeeping gene Gene expression profiling biology.protein Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) |
| Zdroj: | Analytical biochemistry. 408(1) |
| ISSN: | 1096-0309 |
| Popis: | This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene. The effect of reference gene selection on quantitative real-time polymerase chain reaction data interpretation was demonstrated, normalizing the expression data of a selected gene of interest. Thus, GAPDH may be recommended for data normalization in gene expression studies in astrocytomas. Nevertheless, a preliminary validation of reference gene stability is required prior to every study. |
| Databáze: | OpenAIRE |
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