Cohesin interacts with a panoply of splicing factors required for cell cycle progression and genomic organization
| Autor: | William Stafford Noble, William S. Lane, Xiaoyuan He, Todd Waldman, Julia Gerard, Taeyeon Kim, Bogdan Budnik, Jung-Sik Kim, Jie Liu, Zhijun Duan, Brian S. Kim |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Cohesin Cohesin complex RNA-binding protein Biology Chromatin Cell biology Establishment of sister chromatid cohesion 03 medical and health sciences 0302 clinical medicine Prophase RNA splicing biological phenomena cell phenomena and immunity Mitosis 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/325209 |
| Popis: | The cohesin complex regulates sister chromatid cohesion, chromosome organization, gene expression, and DNA repair. Here we report that endogenous human cohesin interacts with a panoply of splicing factors and RNA binding proteins, including diverse components of the U4/U6.U5 tri-snRNP complex and several splicing factors that are commonly mutated in cancer. The interactions are enhanced during mitosis, and the interacting splicing factors and RNA binding proteins follow the cohesin cycle and prophase pathway of regulated interactions with chromatin. Depletion of cohesin-interacting splicing factors results in stereotyped cell cycle arrests and alterations in genomic organization. These data support the hypothesis that splicing factors and RNA binding proteins control cell cycle progression and genomic organization via regulated interactions with cohesin and chromatin.One Sentence SummaryEndogenous tagging reveals that cohesin interacts with diverse chromatin-bound splicing factors that regulate cell cycle progression and genomic organization in human cells. |
| Databáze: | OpenAIRE |
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