In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model
| Autor: | Jae Jun Song, Yoon Young Go, Sung Won Chae, Gi Jung Im, Mukesh Kumar Yadav |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Immunology poly-microbial medicine.disease_cause Microbiology 03 medical and health sciences Immune system In vivo medicine Colonization Original Research biology Pseudomonas aeruginosa Biofilm otitis media methicillin resistant Staphylococcus aureus biochemical phenomena metabolism and nutrition colonization biology.organism_classification Methicillin-resistant Staphylococcus aureus 030104 developmental biology Infectious Diseases biofilms planktonic methicillin resistant Staphylococcusaureus Staphylococcus aureus Bacteria |
| Zdroj: | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY(7) Frontiers in Cellular and Infection Microbiology |
| ISSN: | 2235-2988 |
| Popis: | Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are known to cause biofilm-related infections. MRSA and PA have been frequently isolated from chronically infected wounds, cystic fibrosis, chronic suppurative otitis media (CSOM), and from indwelling medical devices, and these bacteria co-exist; however, their interaction with each-other or with the host is not well known. In this study, we investigated MRSA and PA multi-species biofilm communities in vitro and their interaction with the host during in vivo colonization using an OM rat-model. In-vitro biofilm formation and in-vivo colonization were studied using CV-microtiter plate assay and OM rat-model respectively. The biofilms were viewed under scanning electron microscope and bacteria were enumerated using cfu counts. The differential gene expressions of rat mucosa colonized with single or multi-species of MRSA or PA were studied using RNA-sequencing of total transcriptome. In multi-species in-vitro biofilms PA partially inhibited SA growth. However, no significant inhibition of MRSA was detected during in-vivo colonization of multi-species in rat bullae. A total of 1,797 genes were significantly (p < 0.05) differentially expressed in MRSA or PA or MRSA + PA colonized rat middle ear mucosa with respect to the control. The poly-microbial colonization of MRSA and PA induced the differential expression of a significant number of genes that are involved in immune response, inflammation, signaling, development, and defense; these were not expressed with single species colonization by either MRSA or PA. Genes involved in defense, immune response, inflammatory response, and developmental process were exclusively up-regulated, and genes that are involved in nervous system signaling, development and transmission, regulation of cell growth and development, anatomical and system development, and cell differentiation were down-regulated after multi-species inoculation. These results indicate that poly-microbial colonization induces a host response that is different from that induced by single species infection. |
| Databáze: | OpenAIRE |
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