Topical Deferoxamine Alleviates Skin Injury and Normalizes Atomic Force Microscopy Patterns Following Radiation in a Murine Breast Reconstruction Model
| Autor: | Yekaterina Polyatskaya, Mark M. Banaszak Holl, Jeremy V. Lynn, Kevin M. Urlaub, Noah S. Nelson, Alexis Donneys, Steven R. Buchman, Russell E. Ettinger, Alicia Snider, Geoffrey C. Gurtner |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Administration Topical 030230 surgery Deferoxamine Microscopy Atomic Force Tumor ablation Article Rats Sprague-Dawley 03 medical and health sciences Random Allocation 0302 clinical medicine Fibrosis medicine Animals Skin Chemotherapy Back Atomic force microscopy Skin Injury business.industry Cancer Tissue Expansion Devices medicine.disease Rats Disease Models Animal 030220 oncology & carcinogenesis Surgery Female Radiology Breast reconstruction business medicine.drug |
| Popis: | BACKGROUND: Breast cancer is most commonly managed with a combination of tumor ablation, radiation, and/or chemotherapy. Despite the oncologic benefit of these treatments, the detrimental effect of radiation on surrounding tissue challenges the attainment of ideal breast reconstruction outcomes. The purpose of this study is to determine the ability of topical deferoxamine to reduce cutaneous ulceration and collagen disorganization following radiotherapy in a murine model of expander-based breast reconstruction. METHODS: Female Sprague-Dawley rats (n=15) were divided into three groups: Control (expander), XRT (expander + radiation), and DFO (expander + radiation + deferoxamine). Expanders were placed in a sub-musculocutaneous plane in the right upper back and ultimately filled to 15 cc. Radiation was administered via a fractionated dose of 28 Gy. Deferoxamine was delivered topically for 10 days following radiation. After a 20-day recovery period, skin ulceration and dermal type I collagen organization was analyzed. RESULTS: Compared to Control, the XRT group demonstrated a significant increase in skin ulceration (3.7% vs. 43.3%, p=0.00) and collagen fibril disorganization (26.3% vs. 81.8%, p=0.00). Compared to the XRT group, treatment with topical deferoxamine resulted in a significant reduction in ulceration (43.3% vs. 7.0%, p=0.00) and fibril disorganization (81.8% vs. 15.3%, p=0.00). There were no statistical differences between the Control and DFO groups in skin ulceration or collagen disorganization. CONCLUSIONS: This study suggests topical deferoxamine is capable of reducing skin ulceration and type I collagen fibril disorganization following radiotherapy. This novel application of deferoxamine has potential to enhance expander-based breast reconstruction outcomes and improve quality of life for women suffering the devastating effects of breast cancer. |
| Databáze: | OpenAIRE |
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