Mechanism of o-phenanthroline mediated inhibition of E. coli DNA polymerase I: Formation of template-primer-metal-phenanthroline complexes with resultant loss of catalytic activity
| Autor: | Mukund J. Modak, Kakkudiyil I. Abraham |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
DNA Replication
DNA polymerase Stereochemistry Phenanthroline DNA polymerase II Biophysics Biochemistry Divalent chemistry.chemical_compound Escherichia coli Magnesium Molecular Biology chemistry.chemical_classification Nuclease DNA clamp biology Templates Genetic Cell Biology DNA Polymerase I chemistry biology.protein DNA polymerase I Primer (molecular biology) Copper Phenanthrolines |
| Zdroj: | Biochemical and Biophysical Research Communications. 115:567-576 |
| ISSN: | 0006-291X |
| Popis: | Inhibition of E. coli DNA polymerase I activity by 1,10 phenanthroline in the absence of reducing agents requires a high concentration of inhibitor (1–10 mM) depending upon the template primer used to direct the synthesis. We find that o-phenanthroline, unlike its non-chelating analogue, forms a divalent cation mediated complex with template-primers. Enzyme bound to such complexes is unable to catalyse either polymerization or nuclease functions. |
| Databáze: | OpenAIRE |
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