Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays
| Autor: | Macdara Glynn, David J. Kinahan, Marine Renou, Jens Ducrée, Colette McDonagh, Dirk Kurzbuch, Éanna Bailey, Niamh A. Kilcawley |
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| Rok vydání: | 2016 |
| Předmět: |
food.ingredient
Materials science lcsh:Mechanical engineering and machinery Microfluidics 02 engineering and technology 01 natural sciences Article Lab-on-a-Disc (LoaD) centrifugal microfluidics dissolvable film valving food Cabin pressurization lcsh:TJ1-1570 Electrical and Electronic Engineering business.industry Mechanical Engineering 010401 analytical chemistry Spin rate Electrical engineering Mechanics 021001 nanoscience & nanotechnology Automation 6. Clean water Valve actuator 0104 chemical sciences Baking powder Flow control (fluid) Membrane Control and Systems Engineering 0210 nano-technology business |
| Zdroj: | Micromachines Micromachines; Volume 7; Issue 10; Pages: 175 Micromachines, Vol 7, Iss 10, p 175 (2016) |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi7100175 |
| Popis: | We report a new flow control method for centrifugal microfluidic systems; CO2 is released from on-board stored baking powder upon contact with an ancillary liquid. The elevated pressure generated drives the sample into a dead-end pneumatic chamber sealed by a dissolvable film (DF). This liquid incursion wets and dissolves the DF, thus opening the valve. The activation pressure of the DF valve can be tuned by the geometry of the channel upstream of the DF membrane. Through pneumatic coupling with properly dimensioned disc architecture, we established serial cascading of valves, even at a constant spin rate. Similarly, we demonstrate sequential actuation of valves by dividing the disc into a number of distinct pneumatic chambers (separated by DF membranes). Opening these DFs, typically through arrival of a liquid to that location on a disc, permits pressurization of these chambers. This barrier-based scheme provides robust and strictly ordered valve actuation, which is demonstrated by the automation of a multi-step/multi-reagent DNA-based hybridization assay. |
| Databáze: | OpenAIRE |
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