Dynamic transcriptome analysis and Methoprene-tolerant gene knockdown reveal that juvenile hormone regulates oogenesis and vitellogenin synthesis in Propylea Japonica
| Autor: | Xueke Gao, Jinjie Cui, Guofeng Chang, Jichao Ji, Ningbo HuangFu, Li Dongyang, Xiangzhen Zhu, Li Wang, Zhang Kaixin, Junyu Luo |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Methoprene 01 natural sciences Japonica Transcriptome Vitellogenins 03 medical and health sciences Vitellogenin chemistry.chemical_compound Oogenesis Genetics Humans Gene 030304 developmental biology 0303 health sciences Gene knockdown biology Gene Expression Profiling food and beverages biology.organism_classification Cell biology Juvenile Hormones chemistry Gene Knockdown Techniques Juvenile hormone biology.protein Insect Proteins Female Vitellogenesis 010606 plant biology & botany |
| Zdroj: | Genomics. 113:2877-2889 |
| ISSN: | 0888-7543 |
| Popis: | Propylea japonica has been regarded as one of the most remarkable natural enemies against aphid in China. However, the mechanism of juvenile hormone (JH) regulation of reproduction in P. japonica is still unclear. In this study, we investigated the JH titers of P. japonica and the development of the ovaries. We selected the six different developmental stages of ladybeetle females for transcriptome sequencing. We identified 583 genes involved in insect reproduction regulation, including 107 insect hormone synthesis signaling pathway-related genes and 476 nutrition-sensing signaling pathway-related genes. Transcriptome analysis indicated that a large number JH synthesis- and metabolism-related enzyme genes and some potential nutrient signal sensing- and transduction-related genes were significantly differentially expressed during P. japonica development. We investigated the effects of Met gene silencing on the reproduction of female adults and found that the ovarian maturation, vitellogenesis, and follicular epithelium development in the dsMet treatment group were significantly inhibited. |
| Databáze: | OpenAIRE |
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