Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit
Autor: | R. J. McNICOL, M. Woodhead, Rex M. Brennan, Howard V. Davies, Mark A. Taylor |
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Rok vydání: | 1997 |
Předmět: |
chemistry.chemical_classification
biology Cassis Saxifragaceae food and beverages RNA Bioengineering CDNA Library Construction Ribes biology.organism_classification Polysaccharide Isolation (microbiology) Applied Microbiology and Biotechnology Biochemistry chemistry RNA Plant Fruit Botany RNA extraction Molecular Biology Biotechnology |
Zdroj: | Molecular Biotechnology. 7:1-4 |
ISSN: | 1559-0305 1073-6085 |
DOI: | 10.1007/bf02821541 |
Popis: | Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction. |
Databáze: | OpenAIRE |
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