The Monocyte/IgE Connection: May Polymorphisms in the CD14 Gene Teach Us about IgE Regulation?
| Autor: | Mauro Baldini, Fernando O. Martinez, Donata Vercelli |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Genotype medicine.drug_class CD14 Immunology Lipopolysaccharide Receptors Biology Monoclonal antibody Immunoglobulin E Peripheral blood mononuclear cell Monocytes Immune system medicine Humans Immunology and Allergy Cells Cultured Interleukin 4 Polymorphism Genetic Monocyte Models Immunological CD23 Drug Synergism General Medicine medicine.anatomical_structure biology.protein Interleukin-4 |
| Zdroj: | International Archives of Allergy and Immunology. 124:20-24 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000053658 |
| Popis: | Background: Total IgE levels are known to be under genetic control. Linkage studies have indicated that one or more loci on chromosome 5q may control total IgE, as well as asthma and bronchial hyperresponsiveness to nonspecific stimuli. Our group has undertaken a systematic analysis of chromosome 5q, and has recently characterized five single nucleotide polymorphisms at position –1619, –1359, –1145, –809 and –159 in the promoter of the gene encoding CD14, the myeloid pattern recognition receptor that is critical for efficient innate immune response to lipopolysaccharide (LPS) and bacterial ligands. Methods: Carriers of the major CD14 haplotypes were analyzed for serum levels of IgE and soluble CD14. In vitro IgE synthesis was assessed in peripheral blood mononuclear cells stimulated with IL-4 in the presence or absence of LPS or anti-CD14 monoclonal antibodies. Results: An inverse correlation was found between serum levels of IgE and soluble CD14. On the other hand, in vitro IL-4-dependent IgE synthesis was strongly upregulated by LPS, but suppressed by anti-CD14 monoclonal antibodies. Conclusions: Our results highlight the complex role played by monocytes in IgE regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|