Stimulation of mono (ADP-ribosyl)ation by reduced extracellular calcium levels in human fibroblasts
| Autor: | Robert L. King, Robert T. Dell'Orco, Michael R. Duncan, Myron K. Jacobson, Patrick R. Rankin |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Adenosine Diphosphate Ribose Physiology Clinical Biochemistry chemistry.chemical_element Stimulation Cell Biology Calcium Biology Fibroblasts NAD Stimulation Chemical chemistry.chemical_compound chemistry Biochemistry Biosynthesis Extracellular Homeostasis Humans Calcium depletion NAD+ kinase Extracellular Space Cells Cultured Skin |
| Zdroj: | Journal of cellular physiology. 134(1) |
| ISSN: | 0021-9541 |
| Popis: | Lowering extracellular calcium in cultures of human diploid fibroblast-like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3-aminobenzamide, an inhibitor of ADP-ribosyl transfer reactions. The concentrations of 3-aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP-ribosyl) rather than poly(ADP-ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP-ribosyl)ation is involved in the regulation of cellular Ca2+ levels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text