OVO homologue‐like 1 promotes osteoblast differentiation through BMP2 expression
| Autor: | Young Kwan Sung, Eun-Jung Kim, Won-Gu Jang, Hyeon-Young Min |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Osteocalcin Clinical Biochemistry Bone Morphogenetic Protein 2 Bone morphogenetic protein 2 Cell Line Extracellular matrix Mice 03 medical and health sciences 0302 clinical medicine Osteogenesis medicine Animals RNA Messenger Transcription factor Gene knockdown Osteoblasts biology Chemistry Cell Differentiation Osteoblast Cell Biology DLX5 Alkaline Phosphatase Cell biology DNA-Binding Proteins RUNX2 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis embryonic structures biology.protein Transcription Factors |
| Zdroj: | Journal of Cellular Physiology. 234:11842-11849 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | OVO homologue-like 1 (OVOL1) encodes a C2H2 zinc finger protein and is an evolutionarily conserved gene in mammals. The OVOL1 expression is required for development. However, the function of OVOL1 in bone metabolism remains unreported. Here, we show for the first time the role of OVOL1 in osteoblast differentiation. To determine the role of OVOL1 in osteogenic differentiation, we analyzed OVOL1 expression in the preosteoblastic cell line. OVOL1 messenger RNA expression was induced during osteoblast differentiation. In addition, OVOL1 overexpression enhanced the expression of osteogenic genes including bone morphogenetic protein 2 (BMP2), the inhibitor of DNA binding 1 (Id1), distal-less homeobox 5 (Dlx5), runt-related transcription factor 2 (Runx2), osteocalcin (OC), and alkaline phosphatase (ALP). Moreover, mineralization of the extracellular matrix was increased by OVOL1 overexpression in MC3T3-E1 cells. Furthermore, knockdown of the OVOL1 experiment demonstrated that OVOL1 is required for osteoblast differentiation. Collectively, these results suggest that OVOL1 function as an important regulator of osteoblast differentiation by inducing BMP2 expression in MC3T3-E1 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text