Evaluation of two methods for overcoming the antibiotic carry-over effect

Autor:	Robert H. K. Eng, C. E. Cherubin, Elizabeth N. Tan, S. M. Smith
Rok vydání:	1991
Předmět:	Microbiology (medical) medicine.drug_class Antibiotics Colony Count Microbial Centrifugation General Medicine Microbial Sensitivity Tests Biology biology.organism_classification Microbiology Agar plate Infectious Diseases Evaluation Studies as Topic Carry over effect medicine Bacteria
Zdroj:	European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 10(1)
ISSN:	0934-9723
Popis:	The antibiotic carry-over effect occurs when antibiotic transferred onto the agar plate with the subcultured aliquot is sufficient to inhibit the growth of viable bacteria, and results in a falsely low MBC. This phenomenon was eliminated by widely streaking the transferred aliquot over at least one half of a 100 mm agar plate or by centrifugation and resuspension of the organisms in non-antibiotic-containing media prior to plating. Both methods require more effort than the current method of MBC determination, but can be performed in clinical laboratories and are thus highly recommended in the testing of organisms from endocarditis and meningitis patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aebb049f240bb1f3be0305e041e8c64 https://pubmed.ncbi.nlm.nih.gov/2009876 Zobrazit plný text záznamu Full text from SpringerLink