High-frequency induction of adventitious shoots from hypocotyl segments ofLiquidambar styracifiua L. by thidiazuron

Autor:	M. K. Kim, B. C. Bongarten, Harry E. Sommer, Scott A. Merkle
Rok vydání:	1997
Předmět:	fungi Liquidambar styraciflua Defoliant food and beverages Plant Science General Medicine Root system Biology biology.organism_classification Hypocotyl Tissue culture chemistry.chemical_compound chemistry Shoot Botany Elongation Agronomy and Crop Science Explant culture
Zdroj:	Plant Cell Reports. 16:536-540
ISSN:	1432-203X 0721-7714
DOI:	10.1007/bf01142319
Popis:	The effects of thidiazuron (TDZ) on adventitious bud and shoot formation from hypocotyl segments of sweetgum (Liquidambar styracifiua) were tested alone and in combination with 2,4-dichlorophenoxyacetic acid (2,4-D). The combination of 1 mg/1 TDZ with 0.01 mg/l 2,4-D resulted in the highest frequency of bud production. Lower concentrations of TDZ stimulated shoot production, generating the most shoots at 0.1 mg/1 TDZ with 0.01 mg/1 of 2,4-D. Inhibition of shoot elongation by TDZ was overcome by transferring shoot cultures to a shoot proliferation medium lacking TDZ or containing naphthaleneacetic acid and benzyladenine in addition to TDZ. Shoot production in liquid culture was significantly greater than that in solid culture. Comparisons of in vitro and ex vitro rooting of the adventitious shoots demonstrated that ex vitro rooting produced plants with faster growth rates and more extensive root systems.
Databáze:	OpenAIRE
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