Application of xanthine oxidase-catalyzed luminol chemiluminescence in a mouse interleukin-5 immunoassay
| Autor: | A. J. M. Van Oosterhout, H.A.H. Rongen, Auke Bult, H.M. van der Horst, W.P. van Bennekom |
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| Rok vydání: | 1996 |
| Předmět: |
Xanthine Oxidase
Immunology Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Horseradish peroxidase law.invention Luminol Mice chemistry.chemical_compound law medicine Animals Immunology and Allergy Ferrous Compounds Xanthine oxidase Edetic Acid Horseradish Peroxidase Hypoxanthine Chemiluminescence Detection limit Chromatography biology medicine.diagnostic_test Xanthine chemistry Immunoassay Luminescent Measurements biology.protein Interleukin-5 |
| Zdroj: | Journal of Immunological Methods. 197:161-169 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(96)00131-7 |
| Popis: | A chemiluminescent substrate reagent for use in a sandwich immunoassay for the model antigen mouse interleukin-5 (IL-5) was developed using xanthine oxidase and luminol. Various parameters involved in this chemiluminescent reaction have been studied, including the substrate hypoxanthine, luminol and the Fe(II)-EDTA complex. Addition of the Fe(II)-EDTA complex enhances the chemiluminescence signal considerably. The xanthine oxidase-catalyzed chemiluminescent immunoassay was compared to horseradish peroxidase-linked immunoassays with luminol as chemiluminescent, and tetramethyl benzidine as colorimetric substrate. The detection limit of the xanthine oxidase-luminol assay was found to be about 0.6 pg/ml IL-5, whereas the peroxidase-catalyzed immunoassays have detection limits of about 1.3 (HRP-TMB) and 2.9 pg/ml (HRP-luminol) IL-5. |
| Databáze: | OpenAIRE |
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