Melatonin modifies calmodulin cell levels in MDCK and N1E-115 cell lines and inhibits phosphodiesterase activity in vitro
Autor: | Fernando Antón-Tay, Lourdes Huerto-Delgadillo, Gloria Benítez-King |
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Rok vydání: | 1991 |
Předmět: |
Electrophoresis
endocrine system medicine.medical_specialty Calmodulin Phosphodiesterase Inhibitors Cell Cell Line Melatonin Dogs Internal medicine medicine Animals Molecular Biology biology General Neuroscience Phosphodiesterase Metabolism In vitro medicine.anatomical_structure Endocrinology Mechanism of action Cell culture biology.protein Neurology (clinical) medicine.symptom hormones hormone substitutes and hormone antagonists Developmental Biology medicine.drug |
Zdroj: | Brain research. 557(1-2) |
ISSN: | 0006-8993 |
Popis: | The interaction between melatonin and calmodulin was explored. Calmodulin cell levels in MDCK and N1E-115 cells cultured with 10 −9 M melatonin were increased after 3 days but decreased after 6 days. Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [ 3 H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca 2+ calmodulin shift were observed. The results point out that one of the mechanisms of action of melatonin is a calmodulin-melatonin interaction. |
Databáze: | OpenAIRE |
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