Direct Quantification and Comparison of Intratumoral Hypoxia following Transcatheter Arterial Embolization of VX2 Liver Tumors with Different Diameter Microspheres
| Autor: | Andrew L. Lewis, Carmen Gacchina Johnson, Matthew R. Dreher, Karun Sharma, John Bacher, Genevieve Jacobs, Bradford J. Wood, David L. Woods, Elliot Levy |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Liver tumor Cell Survival medicine.medical_treatment Hemostatics Article Cell Line Tumor medicine Animals Radiology Nuclear Medicine and imaging Embolization Chemoembolization Therapeutic Particle Size Ultrasonography Tumor hypoxia business.industry Arterial Embolization Liver Neoplasms Ultrasound Oxygenation Hypoxia (medical) medicine.disease Cell Hypoxia Microspheres Oxygen Treatment Outcome medicine.anatomical_structure Female Rabbits medicine.symptom Cardiology and Cardiovascular Medicine business Nuclear medicine Artery |
| Zdroj: | J Vasc Interv Radiol |
| ISSN: | 1051-0443 |
| DOI: | 10.1016/j.jvir.2015.06.011 |
| Popis: | PURPOSE: To evaluate the effect of embolic diameter on the achievement of hypoxia following embolization in a preclinical model of liver tumors. MATERIALS AND METHODS: Inoculation of Vx2 tumors in the left liver lobe was performed successfully in 12 new Zealand white rabbits weighing 3.7 kg ± 0.5kg (mean + SD). Tumors were deemed eligible for oxygen measurements when the maximum transverse diameter measured 15mm or more by ultrasound examination. Direct monitoring of oxygenation of implanted rabbit hepatic Vx2 tumors was performed with a fiberoptic electrode during and following transarterial embolization of the proper hepatic artery to angiographic flow stasis with microspheres measuring 70–150, 100–300, or 300–500 microns in diameter. RESULTS: Failure to achieve tumor hypoxia as defined despite angiographic flow stasis was observed in ten of eleven total animals. Embolization microsphere size effect failed to demonstrate a significant trend on hypoxia outcome among the diameters tested, and pair-wise comparisons of different embolic diameter treatment groups showed no difference in hypoxia outcome. All microsphere diameters tested resulted in similar absolute reduction (24.3 ± 18.3, 29.1 ± 1.8, and 19.9 ± 9.3 mm Hg, p=0.66) and percentage drop in oxygen (56.0 ± 23.9, 56.0 ±6.4, and 35.8 ± 20.6 mm Hg, p=0.65) Pairwise comparisons for percent tumor area occupied by embolics showed a significantly reduced fraction for 300–500 μm compared to 70–150 μm diameters (p < 0.05). CONCLUSION: In the rabbit Vx2 liver tumor model, three tested microsphere diameters failed to cause tumor hypoxia measured by a fiberoptic probe sensor according to the adopted hypoxia definitions, although an influence of embolic diameter upon achieved post-embolization hypoxia severity was determined. |
| Databáze: | OpenAIRE |
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