Selected ion monitoring assay for the antidepressant maprotiline

Autor:	David Alkalay, Stephen Carlsen, M. Fred Bartlett, Lakshmi Khemani
Rok vydání:	1979
Předmět:	Anthracenes Heptafluorobutyric anhydride Chemical ionization Chromatography Chemistry Combined use Gas Chromatography-Mass Spectrometry Bioavailability chemistry.chemical_compound Maprotiline medicine Humans Selected ion monitoring Gas chromatography Derivatization Spectroscopy medicine.drug
Zdroj:	Biological Mass Spectrometry. 6:435-438
ISSN:	1096-9888 0306-042X
DOI:	10.1002/bms.1200061006
Popis:	A procedure is described which permits the determination of maprotiline in biological fluids at concentrations ranging from 0.5 to 150 ng ml-1. It relies on the use of N-desmethylclomipramine or isotope labeled maprotiline as the internal standard, on derivatization of the secondary amines with heptafluorobutyric anhydride, and on the combined use of gas chromatography with chemical ionization mass spectrometry and computerized data handling. The assaying procedure is specific, accurate and precise. It is suitable for routine analyses and has sufficient sensitivity to permit monitoring the human blood levels expected from a single therapeutic dose for a week or longer. The method, which can monitor simultaneously isotope labeled and unlabeled maprotiline, can be used to great advantage for reducing variability problems encountered in bioavailability studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ae24a3e5bc92aa2469f04ee0f6a3696 https://doi.org/10.1002/bms.1200061006 Zobrazit plný text záznamu Plný text