Comparison of 25-hydroxyvitamin D3 binding proteins from rat lymph and plasma
| Autor: | Junko Murai, Yukako Yamamoto, Sonoko Masuda, Tadashi Kobayashi, Masako Ishimine, Toshio Okano |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Chromatography medicine.diagnostic_test Chromatofocusing Isoelectric focusing Vitamin D-Binding Protein Size-exclusion chromatography Rats Inbred Strains General Chemistry General Medicine Immunoelectrophoresis High-performance liquid chromatography Rats chemistry.chemical_compound Isoelectric point chemistry Drug Discovery medicine Animals Lymph Sodium dodecyl sulfate Carrier Proteins Chromatography High Pressure Liquid |
| Zdroj: | Chemicalpharmaceutical bulletin. 31(11) |
| ISSN: | 0009-2363 |
| Popis: | The 25-hydroxyvitamin D3 [25 (OH)D3] binding proteins from rat lymph and plasma were isolated and purified by sequential use of gel filtration, affinity and ion exchange chromatographies. The properties of both proteins were examined by high-performance liquid chromatography [HPLC], electrophoretic and immunological methods. The results were as follows. (1) On HPLC analysis using a gel permeation column, the proteins showed the same retention times. (2) On disc and sodium dodecyl sulfate disc gel electrophoresis, they showed the same isoelectric points. (4) On immunoelectrophoresis against rat whole antiserum obtained from rabbit, they showed immunological identity. These results strongly suggest that the 25 (OH)D3 binding proteins from rat lymph and plasma are the same protein. |
| Databáze: | OpenAIRE |
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