Investigating Complex Samples with Molograms of Low-Affinity Binders
| Autor: | Fridolin Treindl, Andreas M. Reichmuth, Katharina Kübrich, Janos Vörös, Dmitry Momotenko, Andreas Frutiger, Christof Fattinger, Yves Blickenstorfer, Volker Gatterdam |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Bioengineering
02 engineering and technology 01 natural sciences immunosignaturing Low affinity diagnostics Humans Instrumentation Fluid Flow and Transfer Processes chemistry.chemical_classification Nonspecific binding Chemistry Process Chemistry and Technology Biomolecule 010401 analytical chemistry Proteins diffractometric sensor 021001 nanoscience & nanotechnology 0104 chemical sciences nonspecific binding focal molography profiling Biochemistry Biomarker (medicine) 0210 nano-technology Biomarkers |
| Zdroj: | ACS Sensors, 6 (3) |
| ISSN: | 2379-3694 |
| DOI: | 10.3929/ethz-b-000478111 |
| Popis: | In vitro diagnostics relies on the quantification of minute amounts of a specific biomolecule, called biomarker, from a biological sample. The majority of clinically relevant biomarkers for conditions beyond infectious diseases are detected by means of binding assays, where target biomarkers bind to a solid phase and are detected by biochemical or physical means. Nonspecifically bound biomolecules, the main source of variation in such assays, need to be washed away in a laborious process, restricting the development of widespread point-of-care diagnostics. Here, we show that a diffractometric assay provides a new, label-free possibility to investigate complex samples, such as blood plasma. A coherently arranged sub-micron pattern, that is, a peptide mologram, is created to demonstrate the insensitivity of this diffractometric assay to the unwanted masking effect of nonspecific interactions. In addition, using an array of low-affinity binders, we also demonstrate the feasibility of molecular profiling of blood plasma in real time and show that individual patients can be differentiated based on the binding kinetics of circulating proteins. ACS Sensors, 6 (3) ISSN:2379-3694 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|