PP2A phosphatase acts upon SAS-5 to ensure centriole formation in C. elegans embryos
| Autor: | Daiju Kitagawa, Pierre Gönczy, Debora Keller, Jérôme Reboul, Jolanta Polanowska, Isabelle Flückiger |
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| Přispěvatelé: | Institut Suisse de Recherches Expérimentales sur le Cancer Lausanne (EPFL) (ISREC - EPFL), Ecole Polytechnique Fédérale de Lausanne (EPFL), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Centriole
Centrosome Duplication Cell Cycle Proteins Gene General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine MESH: Cell Cycle Proteins MESH: Caenorhabditis elegans Basal body Animals Centrosome duplication MESH: Animals Protein Phosphatase 2 Phosphorylation Caenorhabditis elegans Caenorhabditis elegans Proteins 2A Molecular Biology 030304 developmental biology Centrioles 9-Fold Symmetry Protein Spd-2 Genetics 0303 health sciences biology MESH: Centrioles MESH: Protein Phosphatase 2 Cell Biology Protein phosphatase 2 MESH: Caenorhabditis elegans Proteins biology.organism_classification Human-Cells Spindle apparatus Cell biology Centrosome Caenorhabditis-Elegans [SDV.IMM]Life Sciences [q-bio]/Immunology Drosophila Functional-Analysis 030217 neurology & neurosurgery Developmental Biology Centriole assembly |
| Zdroj: | Developmental Cell Developmental Cell, Elsevier, 2011, 20 (4), pp.550-62. ⟨10.1016/j.devcel.2011.02.005⟩ Developmental Cell, 2011, 20 (4), pp.550-62. ⟨10.1016/j.devcel.2011.02.005⟩ |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2011.02.005⟩ |
| Popis: | International audience; Centrosome duplication occurs once per cell cycle and ensures that the two resulting centrosomes assemble a bipolar mitotic spindle. Centriole formation is fundamental for centrosome duplication. In Caenorhabditis elegans, the evolutionarily conserved proteins SPD-2, ZYG-1, SAS-6, SAS-5, and SAS-4 are essential for centriole formation, but how they function is not fully understood. Here, we demonstrate that Protein Phosphatase 2A (PP2A) is also critical for centriole formation in C. elegans embryos. We find that PP2A subunits genetically and physically interact with the SAS-5/SAS-6 complex. Furthermore, we show that PP2A-mediated dephosphorylation promotes centriolar targeting of SAS-5 and ensures SAS-6 delivery to the site of centriole assembly. We find that PP2A is similarly needed for the presence of HsSAS-6 at centrioles and for centriole formation in human cells. These findings lead us to propose that PP2A-mediated loading of SAS-6 proteins is critical at the onset of centriole formation. |
| Databáze: | OpenAIRE |
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