Expression of 5-Lipoxygenase and 5-Lipoxygenase-Activating Protein mRNAs in the Peripheral Blood Leukocytes of Asthmatics
| Autor: | Koichi Hirai, Takeshi Koshino, Saeko Takano, Takako Houjo, Toshiaki Takaishi, Yasuyuki Sano, Koichiro Kudo, Koji Ito, Yutaka Morita, Hidetoshi Kihara, Seiichi Kitani |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male 5-Lipoxygenase-Activating Proteins Biophysics Gene Expression Biochemistry Pathogenesis Leukocyte Count chemistry.chemical_compound Leukocytes medicine Humans RNA Messenger 5-lipoxygenase-activating protein Molecular Biology DNA Primers Asthma Arachidonate 5-Lipoxygenase Base Sequence biology Leukotriene C4 Membrane Proteins Cell Biology Middle Aged medicine.disease Eosinophils chemistry Case-Control Studies Immunology Arachidonate 5-lipoxygenase biology.protein Female Arachidonic acid Bronchoconstriction medicine.symptom Carrier Proteins Leukocyte chemotaxis |
| Zdroj: | Biochemical and Biophysical Research Communications. 247:510-513 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.8789 |
| Popis: | Leukotrienes are a family of arachidonic acid metabolites with potent biological activities such as bronchoconstriction and leukocyte chemotaxis. Recent evidence has demonstrated that the 5-lipoxygenase (5-LO) and 5-lipoxygenase-activating protein (FALP) products of arachidonic acid metabolism, leukotriene C4, D4 and E4, were increased in the serum and the urine of asthmatic patients. Therefore, we examined the expression of 5-LO and FLAP mRNAs in the peripheral blood leukocytes (PBL) of 10 asthmatics and 10 controls. Both 5-LO and FLAP mRNAs of PBL in the asthmatic group were found to be significantly increased compared with those in the control group. These data suggest that up-regulation of 5-LO and FLAP mRNAs might be involved in the increased leukotriene synthesis and play an important role in the pathogenesis of asthma. |
| Databáze: | OpenAIRE |
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