Low molecular weight heparin improves peritoneal ultrafiltration and blocks complement and coagulation
| Autor: | Noushin Yahyapour, Ann Albrektsson, Magnus Braide, Farhan Bazargani |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.drug_class medicine.medical_treatment 030232 urology & nephrology Peritonitis Low molecular weight heparin Vascular permeability 030204 cardiovascular system & hematology Pharmacology Peritoneal dialysis Capillary Permeability Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Thrombin Peritoneum Dialysis Solutions medicine Coagulation (water treatment) Animals Blood Coagulation Neovascularization Pathologic Chemistry Anticoagulants General Medicine Heparin Complement System Proteins Heparin Low-Molecular-Weight medicine.disease Rats Disease Models Animal medicine.anatomical_structure Nephrology Immunology Peritoneal Dialysis medicine.drug |
| Zdroj: | Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis. 25(4) |
| ISSN: | 0896-8608 |
| Popis: | ObjectivesClinical studies have demonstrated that the intraperitoneal (IP) complement and coagulation systems are activated in peritoneal dialysis (PD) patients. In animal models, low molecular weight heparin (LMWH) was seen to inhibit peritoneal angiogenesis, and related compounds have increased ultrafiltration volumes after repeated administration to PD patients. The present study evaluated the effects of LMWH on ultrafiltration, coagulation, and complement activation during a single PD dwell.DesignRats were exposed to a single dose of 20 mL 2.5% glucose-based, filter-sterilized PD fluid, with or without supplementation with LMWH. The PD fluid was administered either as an IP injection or as an infusion through an indwelling catheter. The dwell fluid was analyzed 2 hours later concerning activation of the complement and coagulation cascades, chemotactic activity, neutrophil recruitment, ultrafiltration volume, and glucose and urea concentrations.ResultsExposure to PD fluid induced activation of IP complement [formation of C3a(desArg) and increase of C5a-dependent chemotactic activity] and coagulation (formation of thrombin–antithrombin complex) and recruitment of neutrophils. In the case of IP injection, neutrophil recruitment and complement activation were inhibited by LMWH. In both models, LMWH inhibited thrombin formation, reduced complement-dependent chemotactic activity, and increased the IP fluid volume, indicating an improved ultrafiltration.ConclusionsThe acute inflammatory reaction to PD fluid involves the complement and coagulation cascades. Addition of LMWH to the PD fluid improves ultrafiltration, inhibits formation of thrombin, and potentially blocks C5a activity. The present results motivate further investigations of the IP cascade systems in PD. |
| Databáze: | OpenAIRE |
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