Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples
| Autor: | Tomáš Zima, Marta Kalousová, Kateřina Levová, Erika Gedeonová, Xue Chadtová Song, Jiří Homola, Markéta Bocková |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Pregnancy-associated plasma protein A education Analytical chemistry Metal Nanoparticles macromolecular substances 02 engineering and technology Buffers 01 natural sciences Biochemistry Analytical Chemistry Blood serum Limit of Detection Pregnancy Blood plasma Humans Pregnancy-Associated Plasma Protein-A Surface plasmon resonance Detection limit Chromatography Chemistry 010401 analytical chemistry technology industry and agriculture Surface Plasmon Resonance 021001 nanoscience & nanotechnology Blood proteins 0104 chemical sciences Colloidal gold Female Gold 0210 nano-technology Biosensor |
| Zdroj: | Analytical and Bioanalytical Chemistry. 408:7265-7269 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-016-9664-z |
| Popis: | Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer. |
| Databáze: | OpenAIRE |
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