FSH Stimulation with Short Withdrawal Improves Oocyte Competence in Italian Mediterranean Buffalo (Bubalus bubalis)
Autor: | Georgios Petrovas, G. Zullo, G. Albero, Giorgio Antonio Presicce, Marco Russo, M. A. Kosior, Bianca Gasparrini, Serhat Alkan |
---|---|
Přispěvatelé: | Petrovas, G., Kosior, M. A., Presicce, G. A., Russo, M., Zullo, G., Albero, G., Alkan, S., Gasparrini, B., İÜC, Veteriner Fakültesi, Klinik Bilimler Bölümü |
Rok vydání: | 2020 |
Předmět: |
endocrine system
Buffalo Stimulation Article OPU Andrology IVEP lcsh:Zoology Follicular phase medicine lcsh:QL1-991 Blastocyst lcsh:Veterinary medicine General Veterinary biology Embryo biology.organism_classification Oocyte Coasting Dominant follicle medicine.anatomical_structure lcsh:SF600-1100 Animal Science and Zoology Bubalus hormones hormone substitutes and hormone antagonists |
Zdroj: | Animals : an Open Access Journal from MDPI Animals Volume 10 Issue 11 Animals, Vol 10, Iss 1997, p 1997 (2020) |
ISSN: | 2076-2615 |
Popis: | The aim of this work was to evaluate the efficiency of different FSH doses and FSH coasting times before ovum pick-up (OPU) on follicular growth and oocyte competence in buffalo. Experiment 1 involved two different FSH treatments: 40 mg FSH given three (FSH3) or six (FSH6) times, 2 days after dominant follicle removal were tested, with OPU carried out after 40&ndash 44 h of coasting. In experiment 2, OPU was carried out after FSH6 protocol followed by 28&ndash 32 h (C1), 40&ndash 44 h (C2), or 64&ndash 68 h (C3) of coasting time. Cumulus oocyte complexes (COCs) were classified, in vitro matured, fertilized, and cultured. The results demonstrated that FSH6 increased the total number of follicles, the number and percentages of medium and large follicles, the number and the proportion of good quality oocytes, and the number of grade 1,2 and fast-developing blastocysts compared to the control. C3 decreased the percentage of good quality oocyte and blastocyst rates compared to C1 and C2. A higher percentage of fast blastocysts and average number of grade 1,2 blastocysts was observed in C1 compared to C3, with intermediate values found in C2. The improved efficiency in terms of blastocyst yields suggests the use of FSH6 + C1 protocol for ovarian superstimulation in buffalo. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |