Highly Selective, Naked-Eye, and Trace Discrimination between Perfect-Match and Mismatch Sequences Using a Plasmonic Nanoplatform
| Autor: | Jing Lei, Jingjing Niu, Xiaodong Ding, Cuiyun Yin, Xiaoling Li, Dan Xiao, Yuyang Wu, Cuisong Zhou |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Base Pair Mismatch
High selectivity Human immunodeficiency virus (HIV) Deoxyribozyme Color 02 engineering and technology 010402 general chemistry medicine.disease_cause Sensitivity and Specificity 01 natural sciences Analytical Chemistry medicine Humans Nanotechnology Volume concentration Plasmon business.industry Chemistry HIV DNA Catalytic 021001 nanoscience & nanotechnology Highly selective 0104 chemical sciences G-Quadruplexes DNA Viral Hemin Optoelectronics Naked eye 0210 nano-technology business Nucleic Acid Amplification Techniques |
| Zdroj: | Analytical Chemistry. 90:7371-7376 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | A plasmonic nanoplatform to perform an enzyme-free, naked-eye, and trace discrimination of single-base mutation from fully matched sequence is reported. The nanoplatform showed great potential to enhance catalytic hairpin assembly (CHA) amplification efficiency and biocatalytic activity of hemin/G-quadruplex (DNAzyme). When human immunodeficiency virus (HIV) DNA biomarker was used as the model analyst, a naked-eye detection with high selectivity and high sensitivity down to 10–17 M in whole serum was achieved by observing red-to-blue color change. Single-base mismatch and two-base mismatch were detected at the low concentrations of 10–11 and 10–8 M, respectively. The naked-eye detection based on the enzyme-free plasmonic nanoplatform is expected to have potential applications ranging from quick detection and early diagnostics to point-of-care research. |
| Databáze: | OpenAIRE |
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