CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure
| Autor: | Shaohui Niu, Zhiping Qin, Qing Zhi, Jing Li, En Li, Lihua Zhang, Liqiang Sun, Yuan Li, Jiajia Zhao, Liguo Jian, Haiyan Xiong |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Human Embryonic Stem Cells Cardiomyopathy Germ layer Biology Compound heterozygosity Cell Line 03 medical and health sciences 0302 clinical medicine medicine CRISPR Humans CSRP3 lcsh:QH301-705.5 Heart Failure Dilated cardiomyopathy Cell Biology General Medicine medicine.disease Cell biology 030104 developmental biology lcsh:Biology (General) Heart failure Knockout mouse Female CRISPR-Cas Systems Cardiomyopathies 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 49, Iss, Pp 102077-(2020) |
| ISSN: | 1876-7753 |
| Popis: | The role of muscle LIM protein (MLP), encoded by CSRP3, is not well understood in humans. CSRP3 knockout mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Using CRISPR/Cas9, we generated an MLP deficient human ESC line WAe009-A-41 carrying a compound heterozygous 13 bp deletion/1 bp insertion in the CSRP3 gene. The WAe009-A-41 line exhibited a normal female karyotype (46, XX), expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. MLP expression was not detectable in WAe009-A-41 line. This cell line can be a useful tool for studying the role of CSRP3 in cardiomyopathy and heart failure. |
| Databáze: | OpenAIRE |
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