Neurogranin regulates CaM dynamics at dendritic spines
| Autor: | Nashaat Z. Gerges, Amber Petersen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Dendritic spine
animal structures Calmodulin Dendritic Spines Hippocampus Gene Expression Article law.invention Confocal microscopy law Animals Neurogranin Calcium Signaling Phosphorylation Calcium signaling Multidisciplinary Microscopy Confocal biology Fluorescence recovery after photobleaching Cell biology Rats Synapses biology.protein Protein Binding |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Calmodulin (CaM) plays a key role in synaptic function and plasticity due to its ability to mediate Ca2+ signaling. Therefore, it is essential to understand the dynamics of CaM at dendritic spines. In this study we have explored CaM dynamics using live-cell confocal microscopy and fluorescence recovery after photobleaching (FRAP) to study CaM diffusion. We find that only a small fraction of CaM in dendritic spines is immobile. Furthermore, the diffusion rate of CaM was regulated by neurogranin (Ng), a CaM-binding protein enriched at dendritic spines. Interestingly, Ng did not influence the immobile fraction of CaM at recovery plateau. We have previously shown that Ng enhances synaptic strength in a CaM-dependent manner. Taken together, these data indicate that Ng-mediated enhancement of synaptic strength is due to its ability to target, rather than sequester, CaM within dendritic spines. |
| Databáze: | OpenAIRE |
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