Inhibition of the activity of restriction endonucleases by spermidine and spermine
| Autor: | Malla Kuosmanen, Hannu Pösö |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Polyamine
Spermidine Biophysics EcoRI Spermine Biology Biochemistry chemistry.chemical_compound Endonuclease Plasmid Structural Biology Escherichia coli Putrescine Genetics Molecular Biology DNA Restriction Enzymes Cell Biology Molecular biology Kinetics Restriction enzyme chemistry Plasmid digestion inhibition biology.protein DNA Plasmids |
| Zdroj: | FEBS Letters. 179:17-20 |
| ISSN: | 0014-5793 |
| Popis: | Physiological concentrations (0.5–2.0 mM) of spermidine and spermine were observed to inhibit the digestion in vitro of plasmid pJDB 207 by the restriction endonucleases Bam HI (EC 3.1.23.6), Eco RI (EC 3.2.23.13), Hin dIII (EC 3.1.23.20), Hpa I (EC 3.1.23.23) and Pst I (EC 3.1.23.31). The polyamines protected all the tested restriction sequences of DNA, since the activity of all endonucleases used was strongly inhibited. These results show the need for caution when using polyamines as experimental tools for recombinant DNA chemistry. |
| Databáze: | OpenAIRE |
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