Protein transfer of the costimulatory molecule, B7-2 (CD86), into tumor membrane liposomes as a novel cell-free vaccine
| Autor: | Larry E. Westerman, Peter E. Jensen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Insecta
Immunology CHO Cells Transfection Major histocompatibility complex Cancer Vaccines Cell Line law.invention Mice Immune system Adjuvants Immunologic Antigens CD law Cricetinae Tumor Cells Cultured Animals Immunology and Allergy CD86 Mice Inbred BALB C Liposome Membrane Glycoproteins biology Immunogenicity Recombinant Proteins Membrane Biochemistry Liposomes biology.protein Recombinant DNA Immunization B7-2 Antigen Baculoviridae CD80 |
| Zdroj: | Journal of Immunological Methods. 236:77-87 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/s0022-1759(99)00241-0 |
| Popis: | Several approaches have been taken to enhance the immunogenicity of tumors. Genetically-modified tumors expressing various cytokines, major histocompatibility complex (MHC) molecules, or costimulatory molecules such as B7-1 (CD80) or B7-2 (CD86) can induce tumor-specific immune responses. In the present study, an alternative approach was explored based on direct protein transfer of purified recombinant B7-2 into tumor cell membranes. B7-2 was purified from recombinant baculovirus infected insect cells. Although differentially glycosolyated, the recombinant B7-2 retained the function to costimulate T-cell proliferation. Purified B7-2 was readily incorporated into tumor membranes using a detergent dialysis technique to form unilameller liposomes. The immunogenicity of tumor membrane proteoliposomes was significantly increased by incorporation of B7-2. These findings suggest an alternative method for the introduction of immunostimulatory molecules into tumor membranes to create novel tumor vaccines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect