Influence of Egr-1 in Cardiac Tissue-Derived Mesenchymal Stem Cells in Response to Glucose Variations
Autor: | Bastianelli, Daniela, Siciliano, Camilla, Puca, Rosa, Coccia, Andrea, Murdoch, Colin, Colin, Murdoch, Bordin, Antonella, Mangino, Giorgio, Giulio, Pompilio, Calogero, Antonella, DE FALCO, Elena |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Cell type
Article Subject Cell Population lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology Cell therapy Mice medicine Animals Clonogenic assay education Cells Cultured Cell Proliferation Early Growth Response Protein 1 Mice Knockout education.field_of_study General Immunology and Microbiology Cell growth Myocardium lcsh:R Mesenchymal stem cell Glucose transporter Mesenchymal Stem Cells General Medicine Cell biology body regions Glucose medicine.anatomical_structure Biochemistry Sweetening Agents hormones hormone substitutes and hormone antagonists Research Article |
Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
ISSN: | 2314-6133 2314-6141 |
DOI: | 10.1155/2014/254793 |
Popis: | Mesenchymal stem cells (MSCs) represent a promising cell population for cell therapy and regenerative medicine applications. However, how variations in glucose are perceived by MSC pool is still unclear. Since, glucose metabolism is cell type and tissue dependent, this must be considered when MSCs are derived from alternative sources such as the heart. The zinc finger transcription factor Egr-1 is an important early response gene, likely to play a key role in the glucose-induced response. Our aim was to investigate how short-term changes inin vitroglucose concentrations affect multipotent cardiac tissue-derived MSCs (cMSCs) in a mouse model of Egr-1 KO (Egr-1−/−). Results showed that loss of Egr-1 does not significantly influence cMSC proliferation. In contrast, responses to glucose variations were observed in wt but not in Egr-1−/−cMSCs by clonogenic assay. Phenotype analysis by RT-PCR showed that cMSCs Egr-1−/−lost the ability to regulate the glucose transporters GLUT-1 and GLUT-4 and, as expected, the Egr-1 target genes VEGF, TGFβ-1, and p300. Acetylated protein levels of H3 histone were impaired in Egr-1−/−compared to wt cMSCs. We propose that Egr-1 acts as immediate glucose biological sensor in cMSCs after a short period of stimuli, likely inducing epigenetic modifications. |
Databáze: | OpenAIRE |
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