Accumulation of a 22-kDa protein and its mRNA in the leaves of Raphanus sativus in response to salt stress or water deficit
| Autor: | Gérard Vansuyt, Francine Casse-Delbart, Felicie Lopez, Pierre Fourcroy |
|---|---|
| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Rok vydání: | 1994 |
| Předmět: |
Ecophysiology
0106 biological sciences Physiology Raphanus Plant Science Biology medicine.disease_cause 01 natural sciences 03 medical and health sciences Gene expression Botany medicine Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Proline Northern blot 030304 developmental biology 0303 health sciences RADIS Environmental factor food and beverages Cell Biology General Medicine biology.organism_classification 6. Clean water Salinity Shoot 010606 plant biology & botany |
| Zdroj: | Physiologia Plantarum Physiologia Plantarum, Wiley, 1994, 91, pp.605-614. ⟨10.1111/j.1399-3054.1994.tb02995.x⟩ |
| ISSN: | 1399-3054 0031-9317 |
| Popis: | The response of 10-day-old seedlings of Raphanus sativus L. cv. Fakir to salt stress (100 mM to 200 mM NaCl) was investigated. Three weeks after initiation of salt treatment, the fresh weight of the shoots of salt-treated plants was half that of untreated plants. The salt stress resulted in the accumulation of Na+, preferably in the old leaves. The K+ level was reduced by as much as 50% in the old leaves of NaCl-treated plants, whereas this reduction was only 20–25% in the young leaves. Free proline accumulated in all aerial organs, and the highest levels were found in the young leaves. Patterns of total proteins extracted from the leaves of control or salt-treated plants were compared. The most obvious change concerned a 22-kDa, pl 7.5 polypeptide, which accumulated after exposure of the plants to NaCl. The appearance of this polypeptide was also mediated by a rapid drought stress, and sequencing indicated that it is related to the Kunitz protease inhibitor family. A cDNA clone corresponding to the radish 22-kDa polypeptide was obtained and sequenced. Northern blot analysis showed that salt stress induces a large accumulation of this mRNA in the leaves of radish. |
| Databáze: | OpenAIRE |
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