Elevated levels of circulating CD4(+) CRTh2(+) T cells characterize severe asthma
| Autor: | Cheryl R. Laratta, Dilini Vethanayagam, Harissios Vliagoftis, Lisa Cameron, Mohit Bhutani, Drew Nahirney, Nami Shrestha Palikhe |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Exacerbation Immunology Immunoglobulin E Lymphocyte Activation Severity of Illness Index Flow cytometry Immunophenotyping 03 medical and health sciences Mice 0302 clinical medicine Th2 Cells Adrenal Cortex Hormones Risk Factors T-Lymphocyte Subsets medicine Immunology and Allergy Animals Humans Lymphocyte Count Receptor Asthma medicine.diagnostic_test biology business.industry Gene Expression Profiling GATA3 Chemotaxis Middle Aged medicine.disease Respiratory Function Tests Disease Models Animal 030104 developmental biology Phenotype biology.protein Disease Progression Cytokines Inflammation Mediators business Biomarkers 030215 immunology Signal Transduction |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 46(6) |
| ISSN: | 1365-2222 |
| Popis: | Background Chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and expressed by T cells, eosinophils, basophils, and ILC2 cells. CRTh2 expression by CD4(+) T cells identifies the Th2 subset, and these cells have been characterized as allergen-specific central memory Th2 cells. Recently, activation of the PGD2 -CRTh2 pathway in the lungs was associated with severe asthma. Objective To assess circulating levels of Th2 cells and related mediators in severe asthma and those who experience asthma exacerbations. Methods Peripheral blood cells expressing CRTh2 were characterized by flow cytometry and qRT-PCR. Serum IL-13 and PGD2 were measured by ELISA and compared with asthma severity and tendency to exacerbate. Results Severe asthmatics had more circulating CD4(+) CRTh2(+) T cells, CRTh2 and GATA3 mRNA, and a higher level of serum IL-13 compared to mild/moderate asthmatics. The proportion of CD4(+) CRTh2(+) T cells was associated with lower lung function and was highest in severe asthmatics that exacerbated in the last year. Circulating CD4(+) CRTh2(+) T cells, unlike eosinophils, were positively correlated with inhaled steroid dose. Conclusions and clinical relevance Elevated levels of circulating CD4(+) CRTh2(+) T cells are a feature of severe asthma, despite high-dose corticosteroids. Tracking the systemic level of these cells may help identify type 2 severe asthmatics at risk of exacerbation. |
| Databáze: | OpenAIRE |
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