Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients
| Autor: | Xu-fen Li, Shu Zheng, Wen-zhi Jiang, Dong Xu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Colorectal cancer Keratin-20 Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Cytokeratin Carcinoembryonic antigen Biomarkers Tumor medicine Carcinoma Humans Clinical significance RNA Messenger General Pharmacology Toxicology and Pharmaceutics Stage (cooking) Aged Aged 80 and over General Veterinary biology Reverse Transcriptase Polymerase Chain Reaction business.industry Keratin 20 General Medicine Middle Aged medicine.disease Carcinoembryonic Antigen Biomedicine Real-time polymerase chain reaction biology.protein Keratins Female Colorectal Neoplasms business |
| Zdroj: | Journal of Zhejiang University SCIENCE B. 7:445-451 |
| ISSN: | 1862-1783 1673-1581 |
| DOI: | 10.1631/jzus.2006.b0445 |
| Popis: | This study is aimed at establishing a sensitive approach to detect disseminated tumor cells in peripheral blood and evaluate its clinical significance. A total of 198 blood samples including 168 from colorectal carcinoma (CRC) patients and 30 from healthy volunteers were examined by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) to evaluate the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and cytokeratin 19 (CK19) mRNA. CEA mRNA was detected in 35.8% of patients and 3.3% of controls, CK20 mRNA in 28.3% of patients and 6.7% of controls, and CK19 mRNA in 41.9% of patients and 3.3% of controls. CEA and CK20 mRNA positive ratio increased with the advancing Dukes stages, but there was no significant difference in positive ratio between any two stages (P>0.05). Also, relatively high positive ratio of CEA, CK20 and CK19 mRNA expression was observed in some CRC patients with earlier Dukes stages. A higher positive ratio was obtained when two or three detection markers were combined compared to a single marker. Our study indicates that quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood is a valuable tool for monitoring early stage dissemination of CRC cells in blood circulation. |
| Databáze: | OpenAIRE |
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