Fine mapping of a large-effect QTL conferring Fusarium crown rot resistance on the long arm of chromosome 3B in hexaploid wheat
| Autor: | Catherine Feuillet, Jiri Stiller, Yuming Wei, Zhi Zheng, Qi Feng, Qiang Zhao, John M. Manners, You-Liang Zheng, Chunji Liu, Frédéric Choulet, Guijun Yan, Jian Ma, Bin Han |
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| Přispěvatelé: | Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), University of Western Australia, National Foxtail Millet Improvement Centre, Partenaires INRAE, Sichuan Agricultural University, Shanghai Institute for Biological Sciences, CAS, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institute of Millet Crops, CAS/CSIRO project R-1910-1, International Science & Cooperation Program of China 2015DFA30600, University of Western Australia, Sichuan Agricultural University, China Scholarship Council, China Scholarship Council (CSC), Liu, C. J., The University of Western Australia (UWA), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP) |
| Rok vydání: | 2015 |
| Předmět: |
Quantitative Trait Loci
blé hexaploïde Population Locus (genetics) Plant disease resistance Biology Quantitative trait locus marqueur Chromosomes Plant Genetic linkage Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology education Gene Triticum SSR marker fusarium Disease Resistance Plant Diseases marker 2. Zero hunger chromosome 3B education.field_of_study Vegetal Biology fusarium crown rot qtl Chromosome Mapping food and beverages SSR hexaploid wheat fine mapping co-segregating Chromosome Arm DNA microarray Biologie végétale Research Article Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, BioMed Central, 2015, 16 (850), 7 p. ⟨10.1186/s12864-015-2105-0⟩ BMC Genomics 850 (16), 7 p.. (2015) |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-015-2105-0 |
| Popis: | Background Fusarium crown rot (FCR) is a major cereal disease in semi-arid areas worldwide. Of the various QTL reported, the one on chromosome arm 3BL (Qcrs.cpi-3B) has the largest effect that can be consistently detected in different genetic backgrounds. Nine sets of near isogenic lines (NILs) for this locus were made available in a previous study. To identify markers that could be reliably used in tagging the Qcrs.cpi-3B locus, a NIL-derived population consisting of 774 F10 lines were generated and exploited to assess markers selected from the existing linkage map and generated from sequences of the 3B pseudomolecule. Results This is the first report on fine mapping a QTL conferring FCR resistance in wheat. By three rounds of linkage mapping using the NILs and the NIL-derived population, the Qcrs.cpi-3B locus was mapped to an interval of 0.7 cM covering a physical distance of about 1.5 Mb. Seven markers co-segregating with the locus were developed. This interval contains a total of 63 gene-coding sequences based on the 3B pseudomolecule, and six of them were known to encode disease resistance proteins. Several of the genes in this interval were among those responsive to FCR infection detected in an earlier study. Conclusions The accurate localization of the Qcrs.cpi-3B locus and the development of the markers co-segregating with it should facilitate the incorporation of this large-effect QTL conferring FCR resistance into breeding programs as well as the cloning of the gene(s) underlying the QTL. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-2105-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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