Molecular diagnostics on the toxigenic potential of F usarium spp. plant pathogens
| Autor: | Delfina Popiel, Adam Dawidziuk, Joanna Kaczmarek, Maciej Buśko, Grzegorz Koczyk |
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| Rok vydání: | 2014 |
| Předmět: |
Fusarium
Genes Fungal markers Biology medicine.disease_cause Fumonisins Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology law.invention chemistry.chemical_compound law mycotoxins medicine Mycotoxin Gene Zearalenone Polymerase chain reaction Direct sequencing plant pathology Toxin food and beverages Original Articles General Medicine Molecular diagnostics biology.organism_classification chemistry fungi Trichothecenes Biotechnology |
| Zdroj: | Journal of Applied Microbiology |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | Aims We propose and test an efficient and rapid protocol for the detection of toxigenic Fusarium isolates producing three main types of Fusarium-associated mycotoxins (fumonisins, trichothecenes and zearelanone). Methods and Results The novel approach utilizes partially multiplexed markers based on genes essential for mycotoxin biosynthesis (fumonisin—fum6, fum8; trichothecenes—tri5, tri6; zearalenone, zea2) in Fusarium spp. The protocol has been verified by screening a collection of 96 isolates representing diverse species of filamentous fungi. Each Fusarium isolate was taxonomically identified through both molecular and morphological techniques. The results demonstrate a reliable detection of toxigenic potential for trichothecenes (sensitivity 100%, specificity 95%), zearalenone (sensitivity 100%, specificity 100%) and fumonisins (sensitivity 94%, specificity 88%). Both presence and identity of toxin biosynthetic genes were further confirmed by direct sequencing of amplification products. Conclusions The cross-species-specific PCR markers for key biosynthetic genes provide a sensitive detection of toxigenic fungal isolates, contaminating biological material derived from agricultural fields. Significance and Impact of the Study The conducted study shows that a PCR-based assay of biosynthetic genes is a reliable, cost-effective, early warning system against Fusarium contamination. Its future use as a high-throughput detection strategy complementing chemical assays enables effective targeted application of crop protection products. |
| Databáze: | OpenAIRE |
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