Effect of Poliovirus Double-Stranded RNA on Viral and Host-Cell Protein Synthesis
| Autor: | M. L. Celma, E. Ehrenfeld |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
viruses
RNA-dependent RNA polymerase Endogeny Sulfur Radioisotopes Tritium medicine.disease_cause Cell-free system HeLa Viral Proteins medicine Protein biosynthesis Humans RNA Messenger Messenger RNA Multidisciplinary Cell-Free System biology Poliovirus RNA biology.organism_classification Virology Neoplasm Proteins Kinetics Protein Biosynthesis RNA Viral Female Biological Sciences: Biochemistry HeLa Cells |
| Zdroj: | Proceedings of the National Academy of Sciences. 71:2440-2444 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Cell-free protein-synthesizing systems that initiate on endogenous messenger RNA have been developed from uninfected and poliovirus-infected HeLa cells. Poliovirus double-stranded RNA is an effective inhibitor of protein synthesis in these extracts, and both cell-directed and virus-specific protein synthesis are equally sensitive to the inhibitory action of double-stranded RNA. The concentrations of double-stranded RNA required for inhibition are not achieved in the infected cell at early times after infection when host-cell shut-off occurs, but rather are achieved only late in infection when virus-specific protein synthesis begins to decline. This indicates that double-stranded RNA does not act as a direct agent to inhibit host cell protein synthesis following infection by poliovirus. The possible significance of inhibition by double-stranded RNA of poliovirus-specific protein synthesis is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|