Irradiated Human Chondrocytes Expressing Bone Morphogenetic Protein 2 Promote Healing of Osteoporotic Bone Fracture in Rats
| Autor: | Vivian Yip, Youngsuk Yi, Sally Hwang, Moon Jong Noh, Chae-Lyul Lim, Lillian Yun, Hyeon-Youl Lee, Kun Bok Lee, Jong-Pil Hyun, Kwan Hee Lee, Asli Ayverdi, Kyoung Baek Choi |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
animal structures Transgene Cell- and Tissue-Based Therapy Biomedical Engineering Bone Morphogenetic Protein 2 Mice Nude Enzyme-Linked Immunosorbent Assay Bioengineering Bone healing Biology Biochemistry Bone morphogenetic protein 2 Cell Line Viral vector Rats Sprague-Dawley Biomaterials Andrology Fractures Bone Mice Chondrocytes Osteogenesis medicine Animals Humans Bone formation Wound Healing Rats Surgery Bone morphogenetic protein 7 Healed fractures Gamma Rays embryonic structures NIH 3T3 Cells Osteoporotic bone Female |
| Zdroj: | Tissue Engineering Part A. 15:2853-2863 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2008.0578 |
| Popis: | Bone morphogenetic protein 2 (BMP2) was selected as a transgene to regenerate osteoporotic bone defects after several BMPs were tested using a bone formation study in nude mice. Human chondrocytes were transduced with a BMP2-containing retroviral vector, and single clones were selected. The cells were characterized over numerous passages for growth and BMP2 expression. The single clones were irradiated and tested for viability. BMP2 expression lasted for 3 weeks before dying off completely after approximately 1 month. Irradiated and non-irradiated transduced chondrocytes successfully healed fractures in osteoporotic rats induced by ovariectomy. The osteoinducing effect of irradiated cells was better than that of their non-irradiated counterparts or a chondrocytes-only control. This study showed that delivering BMP2 from the transduced and irradiated chondrocytes could be an effective and safe method of repairing osteoporotic bone fractures. |
| Databáze: | OpenAIRE |
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