Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum
| Autor: | Gail M. Preston, Arantza Rico, Mark H. Bennett, Wei E. Huang, Silvia Forcat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Agroinfiltration Nicotiana tabacum lcsh:Medicine Pseudomonas syringae Plant Biology 01 natural sciences Microbiology Plant Biology/Plant Biochemistry and Physiology 03 medical and health sciences chemistry.chemical_compound Botany Tobacco lcsh:Science 030304 developmental biology Nicotiana 0303 health sciences Multidisciplinary biology Callose Plant Sciences lcsh:R fungi food and beverages Agrobacterium tumefaciens Microbiology/Plant-Biotic Interactions biology.organism_classification Elicitor chemistry Plant Biology/Agricultural Biotechnology lcsh:Q Plant hormone Salicylic Acid Plant Biology/Plant Cell Biology 010606 plant biology & botany Abscisic Acid Research Article Plant Biology/Plant-Biotic Interactions |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 1, p e8977 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Background: Agrobacterium tumefaciens strain GV3101 (pMP90) is widely used in transient gene expression assays, including assays to study pathogen effectors and plant disease resistance mechanisms. However, inoculation of A. tumefaciens GV3101 into Nicotiana tabacum (tobacco) leaves prior to infiltration with pathogenic and non-host strains of Pseudomonas syringae results in suppression of macroscopic symptoms when compared with leaves pre-treated with a buffer control. Methodology/Findings: To gain further insight into the mechanistic basis of symptom suppression by A. tumefaciens we examined the effect of pre-treatment with A. tumefaciens on the growth of P. syringae, the production of the plant signalling molecules salicylic acid (SA) and abscisic acid (ABA), and the presence of callose deposits. Pre-treatment with A. tumefaciens reduced ABA levels, P. syringae multiplication and P. syringae-elicited SA and ABA production, but promoted increased callose deposition. However, pre-treatment with A. tumefaciens did not suppress necrosis or SA production in leaves inoculated with the elicitor HrpZ. Conclusions/Significance: Collectively, these results show that inoculation of N. tabacum leaves with A. tumefaciens alters plant hormone levels and plant defence responses to P. syringae, and demonstrate that researchers should consider the impact of A. tumefaciens on plant signal transduction when using A. tumefaciens-mediated transient expression assays to investigate ABA-regulated processes or pathogenicity and plant defence mechanisms. |
| Databáze: | OpenAIRE |
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