Novel alternatively spliced mRNA (1c) of the protein kinase A RIα subunit is implicated in haploid germ cell specific expression
| Autor: | Sigurd Ørstavik, Kjetil Taskén, Maria K. Dahle, Nils Reinton, Kristin Austlid Taskén |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Untranslated region Five prime untranslated region Somatic cell Cyclic AMP-Dependent Protein Kinase RIalpha Subunit Protein subunit Molecular Sequence Data Biology Cell Fractionation Testis Gene expression Genetics Animals Humans RNA Messenger Northern blot Cloning Molecular Protein kinase A Base Sequence Alternative splicing Cell Biology Blotting Northern Cyclic AMP-Dependent Protein Kinases Spermatozoa Molecular biology Rats Alternative Splicing Protein Subunits Nucleic Acid Conformation 5' Untranslated Regions Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 59:11-16 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.1001 |
| Popis: | By using 5′ RACE on rat testis cDNA we identified three alternatively spliced mRNAs of the RIα subunit of cAMPhdependent protein kinase that differed in their 5′ untranslated regions. Two of these 5′hregions showed similarity with the human RIα exons 1a and 1b, while the third l1cr constituted a novel mRNA splice variant. Northern blot analysis showed that the 1c mRNA was specifically expressed in testis and only in postmeiotic germ cells. In contrast, the RIα 1b and RIα 1a mRNAs were present both in premeiotic germ cells and somatic cells of the testis, and the expression of both RIα 1a and 1b mRNAs were stimulated by cAMP in Sertoli cells. In sperm, the RIα protein was expressed after meiosis, and targeted to various subcellular structures via anchoring proteins. The RIα 1c haploidhspecific mRNA, therefore, may be important for the regulation of RIα expression in sperm. Mol. Reprod. Dev. 59:11–16, 2001. © 2001 WileyhLiss, Inc. |
| Databáze: | OpenAIRE |
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