Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development
| Autor: | Tsung-Lin Hsieh, Chiung-Wen Chang, Hsiang-Yi Wu, Chi-Kuang Yao, Tsai-Ning Li, Gunn-Guang Liou, Shan-Shan Lin, Hsin-Chieh Lin, Ya-Wen Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal structures Podosome Neuromuscular Junction Actin remodeling Biology medicine.disease General Biochemistry Genetics and Molecular Biology Neuromuscular junction Cell biology Postsynaptic cytoskeleton 03 medical and health sciences Dynamin II 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure nervous system Postsynaptic potential medicine Myocyte Humans Centronuclear myopathy 030217 neurology & neurosurgery Cytoskeleton Dynamin |
| Zdroj: | Cell reports. 33(4) |
| ISSN: | 2211-1247 |
| Popis: | Neuromuscular junctions (NMJs) govern efficient neuronal communication with muscle cells, relying on proper architecture of specialized postsynaptic compartments. However, the intrinsic mechanism in muscle cells contributing to NMJ development remains unclear. In this study, we reveal that dynamin-2 (Dyn2) is involved in postsynaptic development of NMJs. Mutations of Dyn2 have been linked to human muscular disorder and centronuclear myopathy (CNM), as well as featured with muscle atrophy and defective NMJs, yet the function of Dyn2 at the postsynaptic membrane is largely unknown. We demonstrate that Dyn2 is enriched at the postsynaptic membrane and regulates NMJ development via actin remodeling. Dyn2 functions as an actin-bundling GTPase to regulate podosome turnover and cytoskeletal organization of the postsynaptic apparatus, and CNM-Dyn2 mutations display abnormal actin remodeling and electrophysiological activity of fly NMJs. Altogether, Dyn2 primarily regulates actin cytoskeleton remodeling and NMJ morphogenesis at the postsynaptic membrane, which is distinct from its endocytosis regulatory role at the presynaptic membrane. |
| Databáze: | OpenAIRE |
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