RNA-protein organization of U1, U5 and U4-U6 small nuclear ribonucleoproteins in HeLa cells
| Autor: | I. Reveillaud, Philippe Jeanteur, Claude Brunel, M.N. Lelay-Taha, Joannes Sri-Widada |
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| Rok vydání: | 1986 |
| Předmět: |
Immunoprecipitation
G protein Centrifugation Isopycnic Biology environment and public health Structural Biology RNA Small Nuclear Humans Nucleotide snRNP Molecular Biology Ribonucleoprotein Differential centrifugation chemistry.chemical_classification Base Sequence urogenital system RNA Chromatography Ion Exchange Ribonucleoproteins Small Nuclear Molecular biology Ribonucleoproteins Biochemistry chemistry Nucleic Acid Conformation Electrophoresis Polyacrylamide Gel Small nuclear RNA HeLa Cells |
| Zdroj: | Journal of Molecular Biology. 189:519-532 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(86)90321-9 |
| Popis: | Small nuclear ribonucleoproteins (snRNPs) containing U1 and U5 snRNAs from HeLa cells have been fractionated using a combination of isopycnic centrifugation in cesium chloride and ion-exchange chromatography on DEAE-Sepharose. The procedure is based on the extreme stability conferred upon snRNPs by Mg2+ enabling them to withstand the very high ionic strength that prevails in cesium chloride. U1 snRNP prepared by this method contains all nine major proteins (68K, A, B, B′, C, D, E, F, G) corresponding to those previously identified by immunoprecipitation and is therefore precipitable by anti-RNP and anti-Sm antibodies. U5 snRNP purified in this way contains the common D to G proteins and is also enriched in a 25 × 103 Mr protein that may be U5 snRNP-specific. The core-resistant U5 snRNA sequence (nucleotide 84 to 3′ OH) covered by D to G proteins is extended by only six nucleotides. A similar situation is seen in U4-U6 snRNP, which we have obtained in a sufficiently pure form to examine protected sequences. However, the core-resistant sequence of U4 (nucleotide 116 to 3′ OH) in U4-U6 snRNP is extended by 37 nucleotides, suggesting that the protein composition of this particle could be more complex than that of U5 snRNP. The ribonucleoprotein organization of snRNPs is summarized and discussed in view of our current knowledge on snRNA sequences protected by proteins. |
| Databáze: | OpenAIRE |
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