Optimized enzymatic synthesis of digestive resistant anomalous isoquercitrin glucosides using amylosucrase and response surface methodology
| Autor: | Cheon-Seok Park, Chan-Su Rha, Dae-Ok Kim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Glycosylation Sucrose Chromatography biology Chemistry General Medicine Applied Microbiology and Biotechnology chemistry.chemical_compound Amylosucrase Nutraceutical Enzyme Glucosides Exponential growth Glucosyltransferases biology.protein Quercetin Glycosyl Deinococcus Response surface methodology Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 105:6931-6941 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-021-11532-3 |
| Popis: | Diverse flavonoid glycosides are present in the plant kingdom. Advanced technologies have been utilized to synthesize glycosyl flavonoids which exhibit good physicochemical characteristics. Previously, novel isoquercitrin (IQ) mono-, di-, and tri-glucosides (IQ-G1′, IQ-G2′, and IQ-G3′; atypical IQ-Gs (IQ-Gap)) were synthesized through the reaction of amylosucrase. Here, the regio-selective transglycosylation yields were predicted using response surface methodology for three variables (glucose donor (sucrose; 100–1500 mM), glucose acceptor (IQ; 100–400 µM), and pH (5.0–8.8)) using 1 unit/mL of enzyme at 45 °C; then, the optima were verified according to the experimental responses. Acidity (pH 5.0) was a major contributor for IQ-G1′ production (> 50%), and high sucrose concentration (1500 mM) limited IQ-G3′ production ( 30%). Time-course production of IQ-Gap showed an exponential growth with different rates. IQ-Gap was stable under the simulated intestinal conditions compared with typical IQ-Gs. Digestive stable IQ-Gap can be effectively synthesized by modulating reaction conditions; thereby, atypical glycosyl products may contribute to the elucidation of nutraceutical potential of flavonoid glycosides. •Predictions of RSM were validated for the regio-selective IQ-G ap production. • Time course changes of IQ-G ap indicate non-processive glycosylation of DGAS. • IQ-G ap exceed typical IQ-G in digestive stability at simulated intestinal condition. |
| Databáze: | OpenAIRE |
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